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standard glass microscopy slides  (Fisher Scientific)

 
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    Fisher Scientific standard glass microscopy slides
    Valvular interstitial cell spreading on aligned K2 hydrogels. (A) Day 1, (B) day 3, and (C) day 7 confocal <t>microscopy</t> images of VICs on (i) pre-gelled, (ii) 1× PB, (iii) 3× PB, and (iv) 5× PB hydrogels (DAPI = blue, F-actin = green; scale bars = 300 μm). All images are maximum intensity projections of Z-stacks that have been cropped and rotated so the direction of K2 fibrous alignment is horizontal. Polar histograms to the right of each image display the Fourier gradient structure tensor calculated using the F-actin channels, where the direction of K2 fibrous alignment is 0°. (D) Day 1, (E) day 3, and (F) day 7 comparisons of (i) resultant vector lengths calculated from Fourier gradient structure tensors (n = 3 images; line at mean; *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA and Dunnett’s multiple comparison tests) and (ii) nuclear angle distributions with respect to the angle of K2 fibrous alignment (n = 3 images with between 294 and 1854 pooled nuclei; center line, box bounds, and whiskers indicate the median, first and third quartiles, and 10 and 90 percentiles, respectively; **P < 0.01, ***P < 0.001, ****P < 0.0001 by Kolmogorov–Smirnov test). (G) Day 3 and (H) day 7 confocal microscopy image stitches of VICs on 1× PB hydrogels (DAPI = blue, F-actin = green; scale bar = 500 μm).
    Standard Glass Microscopy Slides, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/standard+glass+microscopy+slides/pmc11285723-341-5-8?v=Fisher+Scientific
    Average 90 stars, based on 1 article reviews
    standard glass microscopy slides - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Tunable Macroscopic Alignment of Self-Assembling Peptide Nanofibers"

    Article Title: Tunable Macroscopic Alignment of Self-Assembling Peptide Nanofibers

    Journal: ACS nano

    doi: 10.1021/acsnano.4c02030

    Valvular interstitial cell spreading on aligned K2 hydrogels. (A) Day 1, (B) day 3, and (C) day 7 confocal microscopy images of VICs on (i) pre-gelled, (ii) 1× PB, (iii) 3× PB, and (iv) 5× PB hydrogels (DAPI = blue, F-actin = green; scale bars = 300 μm). All images are maximum intensity projections of Z-stacks that have been cropped and rotated so the direction of K2 fibrous alignment is horizontal. Polar histograms to the right of each image display the Fourier gradient structure tensor calculated using the F-actin channels, where the direction of K2 fibrous alignment is 0°. (D) Day 1, (E) day 3, and (F) day 7 comparisons of (i) resultant vector lengths calculated from Fourier gradient structure tensors (n = 3 images; line at mean; *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA and Dunnett’s multiple comparison tests) and (ii) nuclear angle distributions with respect to the angle of K2 fibrous alignment (n = 3 images with between 294 and 1854 pooled nuclei; center line, box bounds, and whiskers indicate the median, first and third quartiles, and 10 and 90 percentiles, respectively; **P < 0.01, ***P < 0.001, ****P < 0.0001 by Kolmogorov–Smirnov test). (G) Day 3 and (H) day 7 confocal microscopy image stitches of VICs on 1× PB hydrogels (DAPI = blue, F-actin = green; scale bar = 500 μm).
    Figure Legend Snippet: Valvular interstitial cell spreading on aligned K2 hydrogels. (A) Day 1, (B) day 3, and (C) day 7 confocal microscopy images of VICs on (i) pre-gelled, (ii) 1× PB, (iii) 3× PB, and (iv) 5× PB hydrogels (DAPI = blue, F-actin = green; scale bars = 300 μm). All images are maximum intensity projections of Z-stacks that have been cropped and rotated so the direction of K2 fibrous alignment is horizontal. Polar histograms to the right of each image display the Fourier gradient structure tensor calculated using the F-actin channels, where the direction of K2 fibrous alignment is 0°. (D) Day 1, (E) day 3, and (F) day 7 comparisons of (i) resultant vector lengths calculated from Fourier gradient structure tensors (n = 3 images; line at mean; *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA and Dunnett’s multiple comparison tests) and (ii) nuclear angle distributions with respect to the angle of K2 fibrous alignment (n = 3 images with between 294 and 1854 pooled nuclei; center line, box bounds, and whiskers indicate the median, first and third quartiles, and 10 and 90 percentiles, respectively; **P < 0.01, ***P < 0.001, ****P < 0.0001 by Kolmogorov–Smirnov test). (G) Day 3 and (H) day 7 confocal microscopy image stitches of VICs on 1× PB hydrogels (DAPI = blue, F-actin = green; scale bar = 500 μm).

    Techniques Used: Confocal Microscopy, Plasmid Preparation, Comparison



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    Valvular interstitial cell spreading on aligned K2 hydrogels. (A) Day 1, (B) day 3, and (C) day 7 confocal <t>microscopy</t> images of VICs on (i) pre-gelled, (ii) 1× PB, (iii) 3× PB, and (iv) 5× PB hydrogels (DAPI = blue, F-actin = green; scale bars = 300 μm). All images are maximum intensity projections of Z-stacks that have been cropped and rotated so the direction of K2 fibrous alignment is horizontal. Polar histograms to the right of each image display the Fourier gradient structure tensor calculated using the F-actin channels, where the direction of K2 fibrous alignment is 0°. (D) Day 1, (E) day 3, and (F) day 7 comparisons of (i) resultant vector lengths calculated from Fourier gradient structure tensors (n = 3 images; line at mean; *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA and Dunnett’s multiple comparison tests) and (ii) nuclear angle distributions with respect to the angle of K2 fibrous alignment (n = 3 images with between 294 and 1854 pooled nuclei; center line, box bounds, and whiskers indicate the median, first and third quartiles, and 10 and 90 percentiles, respectively; **P < 0.01, ***P < 0.001, ****P < 0.0001 by Kolmogorov–Smirnov test). (G) Day 3 and (H) day 7 confocal microscopy image stitches of VICs on 1× PB hydrogels (DAPI = blue, F-actin = green; scale bar = 500 μm).
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    Valvular interstitial cell spreading on aligned K2 hydrogels. (A) Day 1, (B) day 3, and (C) day 7 confocal <t>microscopy</t> images of VICs on (i) pre-gelled, (ii) 1× PB, (iii) 3× PB, and (iv) 5× PB hydrogels (DAPI = blue, F-actin = green; scale bars = 300 μm). All images are maximum intensity projections of Z-stacks that have been cropped and rotated so the direction of K2 fibrous alignment is horizontal. Polar histograms to the right of each image display the Fourier gradient structure tensor calculated using the F-actin channels, where the direction of K2 fibrous alignment is 0°. (D) Day 1, (E) day 3, and (F) day 7 comparisons of (i) resultant vector lengths calculated from Fourier gradient structure tensors (n = 3 images; line at mean; *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA and Dunnett’s multiple comparison tests) and (ii) nuclear angle distributions with respect to the angle of K2 fibrous alignment (n = 3 images with between 294 and 1854 pooled nuclei; center line, box bounds, and whiskers indicate the median, first and third quartiles, and 10 and 90 percentiles, respectively; **P < 0.01, ***P < 0.001, ****P < 0.0001 by Kolmogorov–Smirnov test). (G) Day 3 and (H) day 7 confocal microscopy image stitches of VICs on 1× PB hydrogels (DAPI = blue, F-actin = green; scale bar = 500 μm).
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    Valvular interstitial cell spreading on aligned K2 hydrogels. (A) Day 1, (B) day 3, and (C) day 7 confocal <t>microscopy</t> images of VICs on (i) pre-gelled, (ii) 1× PB, (iii) 3× PB, and (iv) 5× PB hydrogels (DAPI = blue, F-actin = green; scale bars = 300 μm). All images are maximum intensity projections of Z-stacks that have been cropped and rotated so the direction of K2 fibrous alignment is horizontal. Polar histograms to the right of each image display the Fourier gradient structure tensor calculated using the F-actin channels, where the direction of K2 fibrous alignment is 0°. (D) Day 1, (E) day 3, and (F) day 7 comparisons of (i) resultant vector lengths calculated from Fourier gradient structure tensors (n = 3 images; line at mean; *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA and Dunnett’s multiple comparison tests) and (ii) nuclear angle distributions with respect to the angle of K2 fibrous alignment (n = 3 images with between 294 and 1854 pooled nuclei; center line, box bounds, and whiskers indicate the median, first and third quartiles, and 10 and 90 percentiles, respectively; **P < 0.01, ***P < 0.001, ****P < 0.0001 by Kolmogorov–Smirnov test). (G) Day 3 and (H) day 7 confocal microscopy image stitches of VICs on 1× PB hydrogels (DAPI = blue, F-actin = green; scale bar = 500 μm).
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    standard (chambered) microscopy slides or dishes with a glass bottom  (Fisher Scientific)
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    Valvular interstitial cell spreading on aligned K2 hydrogels. (A) Day 1, (B) day 3, and (C) day 7 confocal <t>microscopy</t> images of VICs on (i) pre-gelled, (ii) 1× PB, (iii) 3× PB, and (iv) 5× PB hydrogels (DAPI = blue, F-actin = green; scale bars = 300 μm). All images are maximum intensity projections of Z-stacks that have been cropped and rotated so the direction of K2 fibrous alignment is horizontal. Polar histograms to the right of each image display the Fourier gradient structure tensor calculated using the F-actin channels, where the direction of K2 fibrous alignment is 0°. (D) Day 1, (E) day 3, and (F) day 7 comparisons of (i) resultant vector lengths calculated from Fourier gradient structure tensors (n = 3 images; line at mean; *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA and Dunnett’s multiple comparison tests) and (ii) nuclear angle distributions with respect to the angle of K2 fibrous alignment (n = 3 images with between 294 and 1854 pooled nuclei; center line, box bounds, and whiskers indicate the median, first and third quartiles, and 10 and 90 percentiles, respectively; **P < 0.01, ***P < 0.001, ****P < 0.0001 by Kolmogorov–Smirnov test). (G) Day 3 and (H) day 7 confocal microscopy image stitches of VICs on 1× PB hydrogels (DAPI = blue, F-actin = green; scale bar = 500 μm).
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    Image Search Results


    Valvular interstitial cell spreading on aligned K2 hydrogels. (A) Day 1, (B) day 3, and (C) day 7 confocal microscopy images of VICs on (i) pre-gelled, (ii) 1× PB, (iii) 3× PB, and (iv) 5× PB hydrogels (DAPI = blue, F-actin = green; scale bars = 300 μm). All images are maximum intensity projections of Z-stacks that have been cropped and rotated so the direction of K2 fibrous alignment is horizontal. Polar histograms to the right of each image display the Fourier gradient structure tensor calculated using the F-actin channels, where the direction of K2 fibrous alignment is 0°. (D) Day 1, (E) day 3, and (F) day 7 comparisons of (i) resultant vector lengths calculated from Fourier gradient structure tensors (n = 3 images; line at mean; *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA and Dunnett’s multiple comparison tests) and (ii) nuclear angle distributions with respect to the angle of K2 fibrous alignment (n = 3 images with between 294 and 1854 pooled nuclei; center line, box bounds, and whiskers indicate the median, first and third quartiles, and 10 and 90 percentiles, respectively; **P < 0.01, ***P < 0.001, ****P < 0.0001 by Kolmogorov–Smirnov test). (G) Day 3 and (H) day 7 confocal microscopy image stitches of VICs on 1× PB hydrogels (DAPI = blue, F-actin = green; scale bar = 500 μm).

    Journal: ACS nano

    Article Title: Tunable Macroscopic Alignment of Self-Assembling Peptide Nanofibers

    doi: 10.1021/acsnano.4c02030

    Figure Lengend Snippet: Valvular interstitial cell spreading on aligned K2 hydrogels. (A) Day 1, (B) day 3, and (C) day 7 confocal microscopy images of VICs on (i) pre-gelled, (ii) 1× PB, (iii) 3× PB, and (iv) 5× PB hydrogels (DAPI = blue, F-actin = green; scale bars = 300 μm). All images are maximum intensity projections of Z-stacks that have been cropped and rotated so the direction of K2 fibrous alignment is horizontal. Polar histograms to the right of each image display the Fourier gradient structure tensor calculated using the F-actin channels, where the direction of K2 fibrous alignment is 0°. (D) Day 1, (E) day 3, and (F) day 7 comparisons of (i) resultant vector lengths calculated from Fourier gradient structure tensors (n = 3 images; line at mean; *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA and Dunnett’s multiple comparison tests) and (ii) nuclear angle distributions with respect to the angle of K2 fibrous alignment (n = 3 images with between 294 and 1854 pooled nuclei; center line, box bounds, and whiskers indicate the median, first and third quartiles, and 10 and 90 percentiles, respectively; **P < 0.01, ***P < 0.001, ****P < 0.0001 by Kolmogorov–Smirnov test). (G) Day 3 and (H) day 7 confocal microscopy image stitches of VICs on 1× PB hydrogels (DAPI = blue, F-actin = green; scale bar = 500 μm).

    Article Snippet: Samples were imaged on standard glass microscopy slides (Fisher Scientific, Pittsburgh, PA) at a consistent angle.

    Techniques: Confocal Microscopy, Plasmid Preparation, Comparison